Journal: Molecular Vision

Article Title: A role for aquaporin-4 during induction of form deprivation myopia in chick

doi:

Figure Lengend Snippet: Intensity of AQP4 antibody labeling in the nerve fiber layer of chick. The mean relative fluorescence (on an arbitrary scale of 0 to 255) of AQP4/Alexa 596 conjugated antibodies expressed in the nerve fiber layer of the retinae of age-matched normal control eyes (triangles) and both non-deprived eyes (squares) and fellow eyes that experienced form deprivation (diamonds) is plotted against time from initiation of occlusion of the experimental eye. Note that initially intensity of AQP4 labeling is slightly raised in normal eyes and falls with time and increase in eye size. In contrast, occlusion initially causes significant elevation of labeling intensity particularly in the form-deprived eye, but also in the non-occluded fellow eye, which falls with duration of form-deprivation to almost reach control levels after 4 days. Error bars are 1 standard error.

Article Snippet: Slides were washed three times in PBS before incubation for 1 h at room temperature with the fluorescent secondary antibody, Alexa 596-conjugated goat anti-rabbit IgG (Molecular Probes, Eugene, OR), diluted to 5 µg/ml in blocking solution.

Techniques: Antibody Labeling, Fluorescence, Labeling